abstract：:The identification and characterization of Leishmania are relevant to diagnosis, treatment, eco-epidemiology studies, prophylactic measures and control of the disease. Two strains of Leishmania (MHOM/BR/1971/BH49 and MHOM/BR/1971/BH121), isolated from human cutaneous leishmaniasis, were studied using biological and mo...

journal_title：Acta tropica

authors： Silva Sde O,Wu AA,Evans DA,Vieira LQ,Melo MN

更新日期：2009-12-01 00:00:00

abstract：:Cameroon lacks the capacity for routine Pneumocystis pneumonia (PcP) diagnosis, thus, the prevalence of Cameroonian exposure to this microbe is unknown. It is known that Pneumocystis infecting different mammalian host species represent diverse phylogenetic backgrounds and are now designated as separate species. The hi...

journal_title：Acta tropica

authors： Nkinin SW,Daly KR,Walzer PD,Ndzi ES,Asonganyi T,Respaldiza N,Medrano FJ,Kaneshiro ES

更新日期：2009-11-01 00:00:00

abstract：:The major international guidelines for health research have come a long way in setting standards and providing direction on acceptable practices for the conduct of research. However, the responsibilities of the key players have generally been addressed in a rather uncoordinated manner, and there still remain gaps in a...

journal_title：Acta tropica

authors： Chilengi R

更新日期：2009-11-01 00:00:00

abstract：:In Brazil, there are near 20 genera and almost 120 species of scorpions of which 95% reproduce sexually. Parthenogenetic reproduction, however, may also take place. To gain insight into useful molecular markers in parthenogenetic scorpion species, we studied DNA polymorphism using two molecular approaches: simple sequ...

journal_title：Acta tropica

authors： Scholte RG,Caldeira RL,Simões MC,Stutz WH,Silva LL,Carvalho Odos S,Oliveira G

更新日期：2009-11-01 00:00:00

abstract：:Many societies use locally sourced mosquito nets made from a variety of materials. For protecting against malaria these require regular re-treatment with insecticide. K-O Tab 1-2-3 is a 'dip-it-yourself' long-lasting formulation with time-limited interim recommendation from WHO for treatment of washed white and colour...

journal_title：Acta tropica

authors： Oxborough RM,Weir V,Irish S,Kaur H,N'Guessan R,Boko P,Odjo A,Metonnou C,Yates A,Akogbeto M,Rowland MW

更新日期：2009-10-01 00:00:00

abstract：:Trypanosoma rangeli, a non-pathogenic hemoflagelate that in Central and South America infects humans, shares with Trypanosoma cruzi reservoirs and triatomine vectors, as well as geographical distribution. Recently, we have described in T. rangeli a truncated gene copy belonging to the group II of the trans-sialidase s...

journal_title：Acta tropica

authors： Peña CP,Lander N,Rodríguez E,Crisante G,Añez N,Ramírez JL,Chiurillo MA

更新日期：2009-09-01 00:00:00

abstract：:We report a new endemic zone of cutaneous leishmaniasis (CL) in the central area of the State of Paraná (Municipality of Prudentópolis), in southern Brazil. This region was not previously considered endemic for CL, and this work constitutes the first report of CL endemicity there. Leishmaniasis was confirmed by smear,...

journal_title：Acta tropica

authors： Soccol VT,de Castro EA,Schnell e Schühli G,de Carvalho Y,Marques E,Pereira Ede F,Alcantara Fde S,Machado AM,Kowalthuk W,Membrive N,Luz E

更新日期：2009-09-01 00:00:00

abstract：:The prevalence and associated risk factors for Giardia duodenalis in canine and human populations in Temple communities of Bangkok, Thailand were determined by evaluating three common diagnostic methods utilised to detect Giardia, namely zinc sulphate flotation and microscopy, an immunofluoresence antibody test and ne...

journal_title：Acta tropica

authors： Traub RJ,Inpankaew T,Reid SA,Sutthikornchai C,Sukthana Y,Robertson ID,Thompson RC

更新日期：2009-08-01 00:00:00

abstract：:In trematodiases, shape and size of the fluke eggs shed with faeces are crucial diagnostic features because of their typically reduced intraspecific variability. In fascioliasis, the usual diagnosis during the biliary stage of infection is based on the classification of eggs found in stools, duodenal contents or bile....

journal_title：Acta tropica

authors： Valero MA,Perez-Crespo I,Periago MV,Khoubbane M,Mas-Coma S

更新日期：2009-08-01 00:00:00

abstract：:In this study we standardized a new technical approach in which the target mitochondrial DNA sequence (mtDNA) is amplified using a simple but sensitive PCR method as a tool to detect Plasmodium falciparum and Plasmodium vivax. Specific primers were designed to hybridize with cytochrome c oxidase genes of P. falciparum...

journal_title：Acta tropica

authors： Cunha MG,Medina TS,Oliveira SG,Marinho AN,Póvoa MM,Ribeiro-dos-Santos AK

更新日期：2009-07-01 00:00:00

abstract：:We report results of Trypanosoma cruzi infection and parasite genotypes in the wild Octodon degus and synantropic reservoir Rattus rattus from an endemic area with sylvatic Triatoma infestans as the only detected vector. The infection status was determined by hemi-nested PCR directed to minicircles DNA and genotyping ...

journal_title：Acta tropica

authors： Galuppo S,Bacigalupo A,García A,Ortiz S,Coronado X,Cattan PE,Solari A

更新日期：2009-07-01 00:00:00

abstract：:In all, 40 native triatomine species and subspecies occur in NA, belonging to six genera from the Triatomini (Triatoma, Paratriatoma, Panstrongylus, Dipetalogaster, Belminus, Eratyrus), and one genus from the Rhodniini (represented by one non-native species Rhodnius prolixus, formerly occurring exclusively in domestic...

journal_title：Acta tropica

authors： Ibarra-Cerdeña CN,Sánchez-Cordero V,Townsend Peterson A,Ramsey JM

更新日期：2009-05-01 00:00:00

abstract：:Diagnosis of neurocysticercosis (NCC) is complicated because of the variability in clinical presentations and course of the disease where viability of parasite is a major determinant. The present study describes evaluation of ELISAs using Taenia solium metacestode somatic and excretory-secretory (ES) antigens for dete...

journal_title：Acta tropica

authors： Sahu PS,Parija SC,Narayan SK,Kumar D

更新日期：2009-04-01 00:00:00

abstract：:Genetic variability in the Trypanosoma cruzi I group has recently been revealed in Colombian isolates from humans, reservoirs and vectors. Genomic rearrangements and the polymorphic regions in taxonomic markers, such as the miniexon gene, have led to the development of molecular tools to identify phylogenetic haplotyp...

journal_title：Acta tropica

authors： Falla A,Herrera C,Fajardo A,Montilla M,Vallejo GA,Guhl F

更新日期：2009-04-01 00:00:00

abstract：:Sodium Antimony Gluconate (SAG) is currently used worldwide as the first-line drugs for the treatment of visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) since 1940s. Unfortunately, the resistance of Leishmania parasite to this drug is increasing in several parts of the world. The mechanism of drug resista...

journal_title：Acta tropica

authors： Kumar A,Boggula VR,Sundar S,Shasany AK,Dube A

更新日期：2009-04-01 00:00:00

abstract：:The anthelmintic activity of 11 benzimidazole derivatives (A1-A11) and 2 thioureides N,N'-disubstituted (B1-B2) was determined. Each compound and albendazole was tested in vitro against Toxocara canis larvae and in vivo against Hymenolepis nana adult. Compounds A1-A6 and B1-B2 were designed as albendazole prodrugs. Co...

journal_title：Acta tropica

authors： Márquez-Navarro A,Nogueda-Torres B,Hernández-Campos A,Soria-Arteche O,Castillo R,Rodríguez-Morales S,Yépez-Mulia L,Hernández-Luis F

更新日期：2009-03-01 00:00:00

abstract：:The two major African malaria vectors Anopheles gambiae sensu stricto and An. arabiensis are sibling species that occupy different climatic niches but are frequently found in the same larval habitats. Differences in survival and development of the aquatic larval stages of these species at different temperatures may he...

journal_title：Acta tropica

authors： Kirby MJ,Lindsay SW

更新日期：2009-02-01 00:00:00

abstract：:Anopheles mosquitoes are important candidates for genetic control strategies. However, little is known about sperm quality and quantity as determinants of male reproductive success. In this study, sperm quantity and length variation were assessed in testes of un-irradiated and irradiated Anopheles arabiensis. Male rep...

journal_title：Acta tropica

authors： Helinski ME,Knols BG

更新日期：2009-01-01 00:00:00

abstract：:The involvement of the central nervous system (CNS) by Schistosoma mansoni may or may not cause clinical manifestations. When symptomatic, neuroschistosomiasis mansoni (NSM) is one of the most severe presentations of this infection. The neurological manifestations are due to numerous granulomas grouped in confined are...

journal_title：Acta tropica

authors： Ferrari TC,Moreira PR,Cunha AS

更新日期：2008-11-01 00:00:00

abstract：:Over the last 20 years a great advance has been observed in many aspects of medicine, and the advent of novel imaging techniques is certainly amongst the most important. In schistosomiasis these new methods caused a revolution in the definition of the clinical forms of the disease and in the evaluation of its complica...

journal_title：Acta tropica

authors： Lambertucci JR,dos Santos Silva LC,Andrade LM,de Queiroz LC,Carvalho VT,Voieta I,Antunes CM

更新日期：2008-11-01 00:00:00

abstract：:Plasmodium vivax merozoite surface protein 1 (PvMSP-1) is a leading malaria vaccine candidate. This protein is processed to give rise to various sized fragments during merozoite maturation. Here, we describe the analysis of genetic diversity in the 42 kDa C-terminal part of this protein among 33 Indian P. vivax isolat...

journal_title：Acta tropica

authors： Thakur A,Alam MT,Sharma YD

更新日期：2008-10-01 00:00:00

abstract：:Monitoring and evaluation of programme to eliminate lymphatic filariasis (LF) depends on epidemiological assessment using appropriate indicators. Minimum efforts using reliable tests are necessary to guide the programme managers in decision-making. Impact of Mass Drug Administration (MDA) towards filariasis eliminatio...

journal_title：Acta tropica

authors： Hoti SL,Sharma R,Mary KA,Dhamodharan R,Krishnamoorthy K,Das PK

更新日期：2008-09-01 00:00:00

abstract：:The phlebotomine sand fly Lutzomyia longipalpis takes blood from a variety of wild and domestic animals and transmits Leishmania (Leishmania) infantum chagasi, etiological agent of American visceral leishmaniasis. Blood meal identification in sand flies has depended largely on serological methods but a new protocol de...

journal_title：Acta tropica

authors： Sant'Anna MR,Jones NG,Hindley JA,Mendes-Sousa AF,Dillon RJ,Cavalcante RR,Alexander B,Bates PA

更新日期：2008-09-01 00:00:00

abstract：INTRODUCTION:In areas of low-to-moderate risk of malaria transmission, the World Health Organization recommends parasitic confirmation before treatment. Such areas have usually low budget for health care and malaria diagnosis is mostly based on clinical assumption. Algorithms have been developed to improve health care ...

journal_title：Acta tropica

authors： Périssé AR,Strickland GT

更新日期：2008-09-01 00:00:00

abstract：:Data on soil-transmitted helminth (STH) infections and reinfection among Orang Asli (aborigine) schoolchildren and their nutritional and socioeconomic status were analyzed to investigate the pattern and the possible predictors of STH reinfection. In this longitudinal study, 120 (60 males and 60 females) Orang Asli pri...

journal_title：Acta tropica

authors： Hesham Al-Mekhlafi M,Surin J,Atiya AS,Ariffin WA,Mohammed Mahdy AK,Che Abdullah H

更新日期：2008-08-01 00:00:00

abstract：:Four hundred and forty-eight samples of total blood from wild monkeys living in areas where human autochthonous malaria cases have been reported were screened for the presence of Plasmodium using microscopy and PCR analysis. Samples came from the following distinct ecological areas of Brazil: Atlantic forest (N=140), ...

journal_title：Acta tropica

authors： Duarte AM,Malafronte Rdos S,Cerutti C Jr,Curado I,de Paiva BR,Maeda AY,Yamasaki T,Summa ME,Neves Ddo V,de Oliveira SG,Gomes Ade C

更新日期：2008-08-01 00:00:00

abstract：:The combination piperaquine and dihydroartemisinin is emerging as first line treatment of uncomplicated falciparum malaria in Southeast Asia. The aim of this study was to determine the influence of a standard Vietnamese meal on the single-dose pharmacokinetics of piperaquine when administered in combination with dihyd...

journal_title：Acta tropica

authors： Hai TN,Hietala SF,Van Huong N,Ashton M

更新日期：2008-08-01 00:00:00

abstract：:This study provides the first estimate of the genetic diversity and genotype multiplicity of Plasmodium falciparum infections in symptomatic individuals living in Bangui (Central African Republic, CAR). Three hundred thirty six clinical isolates were used for analysis of parasite population polymorphism and genotyped ...

journal_title：Acta tropica

authors： Dolmazon V,Matsika-Claquin MD,Manirakiza A,Yapou F,Nambot M,Menard D

更新日期：2008-07-01 00:00:00

abstract：:Two high-throughput assays for the identification of members of the Anopheles gambiae sensu lato species complex have recently been reported. These methods, are based on TaqMan single nucleotide polymorphism (SNP) genotyping that enables rapid scoring of mosquito DNA samples in real-time PCR reactions. Unfortunately, ...

journal_title：Acta tropica

authors： Bass C,Williamson MS,Field LM

更新日期：2008-07-01 00:00:00

abstract：:Sm14 and paramyosin are two major Schistosoma mansoni vaccine candidate antigens. Recently, we have identified Sm14 and paramyosin epitopes that are recognized by T cells of resistant individuals living in endemic areas for schistosomiasis. Herein, mice were immunized with these peptides separately or in association i...

journal_title：Acta tropica

authors： Garcia TC,Fonseca CT,Pacifico LG,Durães Fdo V,Marinho FA,Penido ML,Caliari MV,de Melo AL,Pinto HA,Barsante MM,Cunha-Neto E,Oliveira SC

更新日期：2008-06-01 00:00:00

abstract：:The influence of live Brugia malayi parasites and a Sephadex G-200 fraction of the adult parasite extract (BmAFII) on the progression of Leishmania donovani infection was studied. Inbred hamsters were first infected with B. malayi infective 3rd stage larvae (L3), adult worms or microfilariae (mf), and then with L. don...

journal_title：Acta tropica

authors： Murthy PK,Dixit S,Gaur RL,Kumar R,Sahoo MK,Shakya N,Joseph SK,Palne S,Gupta S

更新日期：2008-05-01 00:00:00

abstract：:The salivary glands of insect's vectors are target organs to study the vectors-pathogens interactions. Rhodnius prolixus an important vector of Trypanosoma cruzi can also transmit Trypanosoma rangeli by bite. In the present study we have investigated ecto-phosphatase activity on the surface of R. prolixus salivary gla...

journal_title：Acta tropica

authors： Gomes SA,Fonseca de Souza AL,Kiffer-Moreira T,Dick CF,dos Santos AL,Meyer-Fernandes JR

更新日期：2008-05-01 00:00:00

abstract：:Giardiasis is a major problem in León, Nicaragua, yet despite this no data are available regarding the prevalence of different Giardia genotypes in this area. To address this question, a molecular analysis of Giardia isolates from humans and dogs living in the same area in León, Nicaragua was performed. Giardia isolat...

journal_title：Acta tropica

authors： Lebbad M,Ankarklev J,Tellez A,Leiva B,Andersson JO,Svärd S

更新日期：2008-04-01 00:00:00

abstract：:Manipulating the endogenous immune responses of the mosquito such as temporal and spatial expression of antimicrobial peptides may help in the development of a refractory mosquito, unable to transmit malaria. In mosquito several small antimicrobial peptides are activated locally in the midgut and salivary glands upon ...

journal_title：Acta tropica

authors： Dixit R,Sharma A,Patole MS,Shouche YS

更新日期：2008-04-01 00:00:00

abstract：:"Anomalous", as yet unreported forms of brood capsules (BC) in cystic echinococcosis larvae from humans and sheep are described, thought to be the likely effect of degenerative involution of the parasite. Morphological examination showed an inner laminated layer (LL) in the brood capsules in intimate contact with the ...

journal_title：Acta tropica

authors： Conchedda M,Gabriele F,Bortoletti G

更新日期：2008-03-01 00:00:00

abstract：:We have developed a molecular method using PCR-direct sequencing to identify the infective 3rd juvenile stage of Angiostrongylus cantonensis, a nematode parasite of rodents that can accidentally infect humans and cause eosinophilic meningitis. We demonstrate that the 5' end of the small subunit (SSU) ribosomal (r) RNA...

journal_title：Acta tropica

authors： Fontanilla IK,Wade CM

更新日期：2008-02-01 00:00:00

abstract：:Lyophilized cells (sealed and unsealed) and water dispersible powders (WDPs) of two indigenous isolates of Bacillus thuringiensis var.israelensis were stored at -10, 4, 30 and 40 degrees C for up to 4 years and checked for activity. Lyophilized cells stored in sealed condition at -10, 4 and 30 degrees C and WDPs store...

journal_title：Acta tropica

authors： Manonmani AM,Prabakaran G,Hoti SL

更新日期：2008-02-01 00:00:00

abstract：:Resistance of trypanosomes to melarsoprol is ascribed to reduced uptake of the drug via the P2 nucleoside transporter. The aim of this study was to look for evidence of drug resistance in Trypanosoma brucei gambiense isolates from sleeping sickness patients in Ibba, South Sudan, an area of high melarsoprol failure rat...

journal_title：Acta tropica

authors： Maina N,Maina KJ,Mäser P,Brun R

更新日期：2007-11-01 00:00:00

abstract：:Although, pleural (PT) and disseminated tuberculosis (DT) have been considered as extreme endpoints of the Th1-Th2 immunological spectrum of the Mycobacterium tuberculosis infection, these conditions can occur together. The presence of PT and DT could be explained by (1) PT as primary condition, with progression of HI...

journal_title：Acta tropica

authors： Tuon FF,Tonacio AC,Gryschek RC

更新日期：2007-11-01 00:00:00

abstract：:Dicrocoelium dendriticum (Rudolphi, 1819) and Dicrocoelium hospes (Looss, 1907) are recognised to affect the liver of domestic and wild ruminants. A third species, Dicrocoelium orientalis which was described from musk deer in the Baikal region of the former Soviet Union and re-named to Dicrocoelium chinensis (Sudariko...

journal_title：Acta tropica

authors： Otranto D,Rehbein S,Weigl S,Cantacessi C,Parisi A,Lia RP,Olson PD

更新日期：2007-11-01 00:00:00